Journal:
2004. 12 (2)Authors:
I.A. Akimov, S.V. Benedyk, O.P. Berezovskaya, A.P. SidorenkoAbout authors:
I.A. Akimov, Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine, Kiev, 01601 UkraineAnnotation:
Randomly amplified polymorphic DNA (RAPD) was used to reveal haplotype specificity and genetic variation in populations of the mite Varroa destructor Anderson et Trueman, 2000 infesting managed and feral colonies of honeybee in Ukraine. All specimens of mites collected from Apis mellifera L. colonies in Zakarpats'ka Oblast', Poltavs'ka Oblast' andZhytomyrs'kaOblast' were detected to belong to the Korea haplotype of V. destructor. It was not possible to distinguish mites collected from three subspecies of A. mellifera L., but genetic variation within mite populations in-festing feral honeybees A. mellifera mellifera L. in Polessky reserve was substantially higher com-pared to mite populations infesting both A. mellif-era carpatica Petrov (A. mellifera carnica Polln-ann) and A. mellifera acervorum Skorikov (A. mel-lifera artemisia Engel.).
Bibliography:
Akimov I.A., Benedyk S.V. 2004. [Comparative analy-sis of morphological characters of mite Varroa destructor (Parasiti formes: Varroidae) parasitizing honeybees from hive-logs in Polessky preserve]. Vestnik zoologii, 38 (6): 47-53. [in Russian]
Akimov I.A., Benedyk S.V., Zaloznaya L.M. 2004. Complex analysis of morphological characters of gamasid mite Varroa destructor (Parasitiformes, Varroidae). Vestnik zoologii, 38 (5): 57-66.
Akimov I.A., Grobov O.P., Piletskaya I.V., Barabanova V.V., Yastrebtsov A.V., Gorgol V.T., Zaloznaya L.M., Galaktionov Yu.K., Efimov V.M., Nepomn-yashchikh V.A. 1993. [Honeybeemite Varroajacob-soni]. Kiev: Naukova Dumka. 256 pp. [in Russian]
Akimov I.A., Zaloznaya L.M., Efimov V.M., Galak-tionov Yu.K. 1990. [Seasonal and geographical variation of the morphological characters of the mite Varroa jacobsoni (Parasitiformes, Varroidae): moving of mean values, standard deviations and coefficients of the fluctuating asymmetry], Zoolog-icheskiy Zhurnal, 69 (9): 27-37. [in Russian]
Anderson D.L. 2000. Variation in the parasitic bee mite Varroa jacobsoni Oud. Apidologie,
31: 281-292.
Anderson D.L., Fuchs S. 1998. Two genetically distinct populations of Varroa jacobsoni with contrasting reproductive abilities on Apis mellifera. Journal of Apicultural Research, 37 (2): 69-78.
Anderson D.L., Trueman J.W.H. 2000. Varroa jacobsoni (Acari: Varroidae) is more than one species. Exper-imental and Applied Acarology, 24: 165-189.
Biasiolo A. 1992. Lack of allozyme variability among Varroa mite populations. Experimental and Ap-plied Acarology, 16 (4): 287-294.
De Guzman L.I., RindererT.E. 1999. Identification and comparison of Varroa species infesting honey bees. Apidologie, 30(2-3): 85-95.
De Guzman L.I., Rinderer Т.Е., Stelzer J.A. 1997. DNA evidence of the origin of Varroa jacobsoni Oude-mans in the Americas. Biochemical Genetics, 35 (9/ 10): 327-335.
De Guzman L.I., Rinderer Т.Е., Stelzer J.A., Anderson D.L. 1998. Congruence of RAPD and mitochondrial DNA markers in assessing Varroa jacobsoni geno-types. Journal of Apicultural Research, 37: 49-51.
De Jong D. 1988. Varroa jacobsoni does reproduce in worker cells of Apis cerana in South Korea. Apid-ologie, 19 (3): 241-244.
Delfinado M.D. 1963. Mites of the honeybees in South-East Asia. Journal of Apicultural Research, 2: 113-114.
Delfinado-Baker M. D. 1988. Variability and biotypes of Varroa jacobsoni Oudemans. American Bee Journal, 128: 567-568.
Delfinado-Baker M.D., Houck M.A. 1989. Geographic variation in Varroa jacobsoni (Acari, Varroidae): application of multivariate morphometric tech-niques. Apidologie, 20: 345-358.
Garcia Fernandez P. 1997. Influence of the environment and the host on parasitization by Varroa jacobsoni Oud. Cahiers-Options-Mediterraneennes, 21: 33-47.
GrobovO.P. 1981. [The importance of species and some racial characteristics of the honey bee for varroato-sis]. In: Varroatoz pchel. Moscow, Nauka, pp. 23 - 27. [in Russian]
Issa M.R.C. 1989. Enzyme patterns in Varroa and Apis from Brazil and Germany. Apidologie, 20 (6): 506-508.
Koeniger N., Koeniger G., Wijagunasekara N.H.P. 1981. Beobachtungen liber die Anpassung von Varroa jacobsoni an ihren naturlichen Wirt Apis cerana in Srilanka. Apidologie,
12 (1): 37-40.
Kraus В., Hunt G. 1995. Differentiation of Varroa jacobsoni Oud. populations by random amplifica-tion of polymorphic DNA (RAPD). Apidologie, 26 (4): 283-290.
Kuznetzov V.N., Proshchlykin M.Yu. 2004. [Chinese wax bee Apis cerana cerana F. (Hymenoptera, Apidae) in the south of the Russian Far East]. Vladivostok. 56 pp.[in Russian]
Moritz R., Hanel H. 1984. Restricted development of the parasitic mite Varroa jacobsoni Oud. in the Cape honeybee Apis mellifera capensis Esch. Zeitschrift fur angewandte Entomologie, 97 (1): 91-95.
Morse R.A., Gonsalves L.S. 1979. Varroa disease, a theat to world beekeeping. Gleanings in Bee Cul-ture, 107 (4): 179-181, 202.
Piletskaya I. 2002. Varroa invasion of the forestry bees from the logs of Polessky preserve. In: XXXIX Naukowej Konferencji Pszczelarskiej: Materialy z Konferencji (12-13 marca 2002, Pulawy). Pulawy, p. 57-58.
Piletskaya I.V., Zhila S.M. 2003. [The biology and log colony population dynamics of the honeybees in Polessky nature reserve]. Vestnik zoologii, Supple-ment 16: 98-104. [in Russian]
Rosenkranz P., Engels W. 1994. Infertility of Varroa jacobsoni females after invasion into Apis mellifera worker brood as a tolerance factor against varroato-sis. Apidologie, 25(4): 402^11 .
Sambrook J., Fritisch E.F., Maniatis T. 1989. Molecular cloning: Laboratory manual. New York: Cold Spring Harbor Univ. Press., 1962 p.