EVALUATION OF THE EFFICACY OF DIFFERENT ACARICIDES AGAINST VARROA DESTRUCTOR ON APIS MELLIFERA INTERMISSA IN ALGERIA

About authors:

Adjlane N., Department of Biology, Faculty of Science, M’hamed Bougara University of Boumerdes, Algeria
Haddad N., National Centre for Agricultural Research and Extension, Bee Research Department, P.O. Box 639, Baq’a 19381, Jordan

Tarek O., Institut technique des élevages, Baba Ali, Alger, Algérie

Annotation:

Varroa mite has become a major concern of beekeepers in Algeria since the discovery of the first cases of infestation in the year 1982. The objective of this study was to test different registered chemicals aside with those prepared by beekeepers. The experiment was conducted on 50 Apis meliffera intermissa colonies in a commercial apiary and kept in standard Langstroth hives. Among the products which are approved in Algeria and tested in our experiment are Bayvarol which recorded the highest efficiency rate (91.62%), followed by Apivar (86.50%) and then Apistan (77.75%). Traditional preparations treatments had very low efficiency where it was only: 39.37% for amitraz and 44.21% for tau-fluvalinate (Mavrik). Our study showed a reduction in the efficiency of commercial products (Apistan, Bayvarol and Apivar) and a very low efficiency for amitraz and Mavrik. Such results prove the high demand of searching for more effective treatments against Varroa.

Bibliography:

Adjlane, N., Doumandji, S.E. and Haddad, N. 2012.
Situation de l’apiculture en Algérie: facteurs me-
naçant la survie des colonies d’abeilles locales
Apis mellifera intermissa. Cah Agric, 21: 235–
241; doi: 10.1684/agr.2012.0566

Adjlane, N. and Doumandji, S.E. 2011. La varroase:
Biologie, diagnostic et traitement. Situation actu-
elle de la varroase en Algérie. Revue Pratique Vé-
térinaire, 9: 8–11.

Akkaya, A. and Vurusaner, C. 1996. Field experiment
to determine the efficacy of flumethrin (Bayvarol-
Strips) and fluvalinate (Apistan Strips) against
varroatosis of the honey bee colonies. Acta Para-
sitologica Turcica, 20 (3–4): 457–460.

Akkaya, A. and Vurusaner, C. 1997. Field experiment
to determine the efficacy of flumethrin and cou-
maphos against varroasis according to the state of
the honey bee colonies. Acta Parasitologica Tur-
cica, 21 (1): 83–86.

Al-ghamdi, A. 2007. Evaluation of the relative efficacy
of different acaricides against Varroa destructor
on apis mellifera carnica. Annals of agricultural
science Cairo, 52 (2): 501.

Alloui, N., Boucherit, M.R. and Nouicer, F. 2002. Ef-
fect of flumethrin on Varroa destructor in honey
bee colonies. Bulletin of the Veterinary Institute in
Pulawy, 46: 233–237.

Anderson, D.L. and Trueman, J.W.H. 2000. Varroa ja-
cobsoni (Acari: Varroidae) is more than one spe-
cies. Exp. Appl. Acarolology, 24: 165–189.

Ball, B.V. 1993. The damaging effects of Varroa ja-
cobsoni, pp. 9–16. In: A. Matheson (Ed.). Living
Varroa, Ed. Internati. Bee res. Associate., Cardiff,
325 p.

Ball, B.V. and Allen, M.F. 1988. The prevalence of
pathogens in honey bee (Apis mellifera) colonies
infested with the parasitic mite Varroa jacobsoni.
Ann. Appl. Biol., 113: 237–244.

Beetsma, J., Boot, W.J. and Calis, J. 1999. Invasion be-
haviour of Varroa jacobsoni Oud from bees into
Brood cells. Apidologie, 30: 125–140.

Boecking, O. and Genersch, E. 2008. Varroosis — the
ongoing crisis in beekeeping. J. Verbr. Lebensm.,
2: 221–228.

Bowen-Walker, P.L. and Gunn, A. 2001. The effect of
the ectoparasitic mite, Varroa destructor on adult
worker honeybee (Apis mellifera) emergence
weights, water, protein, carbohydrate, and lipid
levels. Entomol. Exp. Appl., 101 (3): 101–112.

Di Prisco, G., Pennacchio, F., Caprio, E., Boncristiani,
H.F. JR., Evans, J.D., and Chen, Y. 2011. Varroa
destructor is an effective vector of Israeli acute
paralysis virus in the honeybee Apis mellifera. J.
Gen. Virol., 92: 151–155.

Elzen, P.J., Baxter, J.R., Spivak, M., and Wilson, W.T.
1999. Amitraz resistance in Varroa: New discov-
ery in North America. Am. Bee J., 139: 362.

Elzen, P.J., Eischen, F.A., Baxter, J.R., Pettis, J., Elzen,
G.W., and Wison, W.T. 1988. Fluvalinate resis-
tance in Varroa jacobsoni from several geographic
locations. Am. Bee J., 138: 674–676.

Elzen, P.J., Eischen J.B., and Baxter, J.B. 1998. Flu-
valinate Resistance in Varroa jacobsoni from sev-
eral Geographic Locations. Am Bee J., 138: 674–
676.

Elzen, P.J. and Westervelt, D. 2002. Detection of cou-
maphos Resistance in Varroa destructor in Flori-
da. Am. Bee J., 142: 291–292.

Faucon, J-P., Drajnudel, P., and Fleché, C.1995. Mise
en évidence d’une diminution de l’efficacité de
l’Apistan utilisé contre la varroase de l’abeille.
Apidologie, 26: 291–296.

Faucon, J.P., Drajnudel, P., Chauzat, M.P., and Aubert,
M. 2007. Contrôle de l’efficacité du médicament
APIVAR ND contre Varroa destructor, parasite de
l’abeille domestique. Revue Méd. Vét., 2007, 158
(6): 283–290.

Feldlaufer, M., Pettis, J., Kochansky, J., and Shimanu-
ki, H. 1997. A gel formulation of formic acid for
the control of control of parasitic mites of honey
bee. Am. Bee J., 137: 661–663.

Floris, I., Satta, A., Garau, V.L., Melis, M., Cabras, P.,
and Aloul, N. 2001. Effectiveness, persistence,
and residue of amitraz plastic strips in the apiary
control of Varroa destructor. Apidologie, 32: 577–
585.

Fries, I. 2005. Economic threshold for Varroa jacobso-
ni Oud. in the southeastern USA. Microbial. Ecol-
ogy, 50: 369–374.

Garcia-Salinas, M., Ferre, M., Latorre, E., Monero, C.,
Castillo, J., Lucientes, J., and Peribanez, M., 2006.
Detection of fluvalinate resistance in Varroa de-
structor in Spanish apiaries. J. Apicult. Res., 45:
101–105.

Gregorc, A. and Skerl, M.I.S. 2007. Combating Varroa
destructor in honeybee colonies using flumethrin
or fluvalinate. Acta Veterinaria Brno, 76: 309–
314.

Guzman-Novoa, E., Eccles, L., Calvete, Y., Mcgowen,
J., Kelly, P.G., and Corra-Benitez, A. 2010. Varroa
destructor is the main culprit for the death and re-
duced populations of overwintered honey bee
(Apis mellifera) colonies in Ontario, Canada. Api-
dologie, 41: 443–450.

Haddad, N., Evans, J., Pettis, J., and Migdali, H. 2007.
Genetic structure of Varroa mite populations in A.
mellifera syriaca. Advances in Environmental Bi-
ology, 1 (1): 1–3.

Jelinski, M. 1993. Effectiveness of the preparation of
Bayvarol-Strips (R) in control of Varroa jacobsoni
mites. Wiad Parazytol., 39: 411–414.

Kochansky, J. 2000. Synthesis of (Diethyl-d10) Cou-
maphos and related Compounds. J. Agric. Food.
Chem, 48: 2826–2828.

Lodesani, M., Colombo, M., and Spreafico, M. 1995.
Ineffectiveness of Apistan treatment against the
mite Varroa jacobsoni Oud. in several districts of
Lombardy (Italy). Apidologie, 26: 67–72.

Lodesani, M. and Costa, M. 2005. Limits of chemo-
therapy in beekeeping: development of resistance
and the problem of residues. Bee World, 86: 102–
109.

Londzin, W. and Sledzinsky, B. 1996. Resistance of
honey bee parasitic mite Varroa jacobsoni to var-
roacide preparates containing tau-fluvalinate. Me-
dicina Weterynaryjna, 52: 526–528.

Loucif-ayad, W., Aribi, N., Smagghe, G., and Soltani,
N. 2010. Comparative effectiveness of some aca-
ricides used to control Varroa destructor (Me-
sostigmata: Varroidae) in Algeria. African Ento-
mology, 18 (2): 259–266.

Mathieu, L. and Faucon, J.P. 2000. Changes in the re-
sponse time for Varroa jacobsoni exposed to ami-
traz. J. of Apicultural Research, 39: 155–158.

Milani, N. and Della Vedova, G. 2002. Decline in the
proportion of mites resistant to fluvalinate in a
population of Varroa destructor not treated with
pyrethroids. Apidologie, 33: 417–422.

Milani, N. 1999. The resistance of Varroa jacobsoni
Oud to acaricides. Apidologie, 30: 229–234.

Pileckas, V. and Klimas, R. 2011. The evaluation of ef-
ficiency of some preparations needed to decimate
Varroa destructor parasites. Acta Biol. Universit.
Daugavpil., 11 (1): 101–105.

Sammataro, D., Untalan, P., Guerro, F., and Finley, J.
2005. The resistance of Varroa mites (Acari: Var-
roidae) to acaricides and the presence of esterase.
Int. J. Acarol., 31 (1): 67–74.

Shahrouzi, R. 2009. The Efficacy of Apivar® and Bay-
varol® and CheckMite+® in the Control of Var-
roa destructor in Iran.
http://www.apiservices.com/articles/us/efficacy_of_
bayvarol.pdf

Rodriguez-Dehaibes, S.R., Otero-Colina, G., Pardio
Sedas, V., and Villanueva Jimenez, J.A. 2005. Re-
sistance to amitraz and flumethrin in Varroa de-
structor populations from Veracruz, Mexico.
Journal of Apicultural Research, 44: 124–125.

Semkiw, P., Skubida, A. and Pohorecka, K. 2013. The
amitraz strips efficacy in control of Varroa de-
structor after many years application of amitraz in
apiaries. Journal of Apicultural Science, 57 (1):
107–121.

Smodiš Škerl, M.I., Nakrst, M., Žvokelj, L., and Grego-
rc, A. 2011. The acaricidal effect of flumethrin,
oxalic acid and amitraz against Varroa destructor
in honey bee (Apis mellifera carnica) colonies.
Acta Vet. Brno, 80: 051–056; doi:10.2754/
avb201180010051.

Spreafico, M., Eordegh, F.R., Bernardinelli, I., and Co-
lombo, M. 2001. First detection of strains of Var-
roa destructor resistant to coumaphos results of
laboratory tests and field trials. Apidologie, 32:
49–55.

Trouiller, J. 1997. Résistance du Varroa au fluvalinate:
résultats des campagnes de détection de 1996. Re-
vue Française d’Apiculture, 571: 115–117.

Watkins, M. 1997. Resistance and its relevance to bee-
keeping. Bee World, 78: 15–22.

Vandame, R., Colin, M.E., Belzunces, L.P., and Jour-
dan, P. 1995. Resistance de Varroa au fluvalinate.
Le Carnet Européen, 3: 5–11.

Yang, X. and Cox-Foster, D.L. 2005. Impact of an ec-
toparasite on the immunity and pathology of an
invertebrate: evidence for host immunosuppres-
sion and viral amplification. Proc. Nat. Acad. Sci.,
102: 7470–7475.